Metaphase II karyoplast transfer from human in-vitro matured oocytes to enuclueated mature oocytes
نویسندگان
چکیده
منابع مشابه
Ultrastructure of in vitro Matured Human Oocytes
BACKGROUND Approximately 20% of recovered oocytes are immature and discarded in intracytoplasmic sperm injection (ICSI) procedures. These oocytes represent a potential resource for both clinical and basic science application. OBJECTIVE The aim of this study was to evaluate the ultrastructure architecture of in vitro matured human oocytes using transmission electron microscopy (TEM). MATERIA...
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Background: The meiotic spindle plays an important role in the oocyte during chromosome alignment and separation at meiosis. The zona pellucid (ZP) is a dynamic matrix composed of filaments with the properties that might reflect the history of oocyte cytoplasmic maturation. Since, spindle and ZP in living oocytes are highly birefringent, their structures can be viewed noninvasively by using a P...
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Ooplasmic transplantation aimed at restoring normal growth in developmentally compromised oocytes and embryos was evaluated in seven couples (eight cycles) with multiple implantation failures. Two approaches were investigated to transfer ooplasm from donor eggs at metaphase II (MII) stage into patient MII eggs: (i) electrofusion of a ooplasmic donor fragment into each patient egg (three cycles)...
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BACKGROUND Somatic cell nuclear transfer (SCNT) involves the transfer of somatic cell nuclei into enucleated oocytes. Because human in vivo matured oocytes are scarcely available, we investigated whether in vitro matured (IVM) germinal vesicle (GV) oocytes could also support preimplantation development of human cloned embryos. METHODS Three groups were used for SCNT: in vitro matured GV oocyt...
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BACKGROUND Concerns regarding the safety of ICSI have been intensified recently due to increased risk of birth defects in ICSI born children. Although fertilization rate is significantly higher in ICSI cycles, studies have failed to demonstrate the benefits of ICSI in improving the pregnancy rate. Poor technical skill, and suboptimal in vitro conditions may account for the ICSI results however,...
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ژورنال
عنوان ژورنال: Reproductive BioMedicine Online
سال: 2009
ISSN: 1472-6483
DOI: 10.1016/j.rbmo.2009.06.004